Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

With the advent of molecular technology, several isothermal techniques for rapid detection zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology rapid, sensitive, and economical pathogens. RPA has advantage being able to be implemented in field settings, because method requires minimal sample preparation performed at a constant low temperature (37–42°C). rapidly promising tool detection, prevention, control diseases. This article discusses principles its derivatives, including coupled with lateral flow testing (RPA-LF), real-time fluorescence RPA, electrochemical flocculation their applications